Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Dec 15;27(25):3991–4001. doi: 10.1091/mbc.E16-08-0563

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 Falk et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

PMC Copyright notice

FIGURE 2: — SPoC mutants retain some checkpoint activity. dyn1-AID kar9Δ (A35707), dyn1-AID kar9Δ kin4Δ (A35603), dyn1-AID kar9Δ rts1Δ (A37483), dyn1-AID kar9Δ bmh1Δ (A36544), dyn1-AID kar9Δ swe1Δ (A35146), and dyn1-AID kar9Δ bub2Δ (A36082) cells carrying GFP-tagged α-tubulin were grown in YEPD medium and arrested in the G1 phase of the cell cycle with 10 μg/ml α-factor pheromone. Cells were released into the cell cycle in YEPD medium and then monitored by live-cell microscopy in a Y04C flow cell. Depletion of dyn1-AID was induced in the flow cell with 100 μM auxin in synthetic complete medium. (A) Fraction of cells that become multinucleate. Number of nuclei was determined upon completion of the first cell cycle after factor release (n = 100). Cells that inappropriately exited from mitosis in the mother cell compartment and hence harbored two nuclei were scored as “multinucleate.” Cells that either arrested permanently with mispositioned spindles in the mother cell compartment or exited mitosis with aligned spindles were scores as “euploid.” (B, C) Anaphase duration was determined in cells that exit from mitosis with mispositioned (B) or correctly positioned (C) spindles. Sample sizes for B: dyn1-AID kar9Δ, 29; dyn1-AID kar9Δ kin4, 31; dyn1-AID kar9Δ rts1Δ, 30; dyn1-AID kar9Δ bmh1Δ, 12; dyn1-AID kar9Δ swe1Δ, 16; and dyn1-AID kar9Δ bub2Δ, 30. The p value in B is <0.05 (two-sided t test). Sample sizes for C: dyn1-AID kar9Δ, 18; dyn1-AID kar9Δ kin4, 20; dyn1-AID kar9Δ rts1Δ, 17; dyn1-AID kar9Δ bmh1Δ, 20; dyn1-AID kar9Δ swe1Δ, 20; and dyn1-AID kar9Δ bub2Δ, 19.