Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Dec 15;27(25):3991–4001. doi: 10.1091/mbc.E16-08-0563

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 Falk et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

PMC Copyright notice

FIGURE 6: — Mislocalizing Lte1 to the mother cell compartment bypasses the SPoC even in the absence of the FEAR. (A) The percentage of multinucleate cells was assessed in dyn1-AID kar9Δ (A35707), dyn1-AID kar9Δ kin4Δ (A35603), dyn1-AID kar9Δ bub2Δ (A36082), and dyn1-AID kar9Δ LTE1-8N (A36357) strains. Cells were cultured as in Figure 2. Spindle mispositioning was scored as in Figure 2A (n = 100). (B) Analysis of anaphase duration in cells that inappropriately exit from mitosis in the mother cell compartment. Cells from A were reanalyzed, and anaphase length was determined by spindle morphology (n = 22). (C) The percentage of multinucleate cells was assessed in dyn1-AID kar9Δ (A35707), dyn1-AID kar9Δ kin4Δ (A35603), dyn1-AID kar9Δ spo12 (A35700), dyn1-AID kar9Δ LTE1-8N (A36357), dyn1-AID kar9Δ LTE1-8N spo12Δ (A36358), dyn1-AID kar9Δ spo12Δ kin4Δ (A36048), dyn1-AID kar9Δ kin4Δ LTE1-8N (A36268), and dyn1-AID kar9Δ LTE1-8N kin4 Δ spo12Δ (A29611) strains. Cells were cultured as in Figure 2. Spindle mispositioning was scored as in Figure 2A (n = 100). Note that the anaphase duration of cells with aligned spindles in the LTE1-8N spo12Δ strain (A36358) was delayed in comparison to spo12Δ cells. This is most likely due to the fact that Lte1-8N is expressed off of its endogenous promoter and yet is distributed on both cortices. Therefore Lte1-8N is essentially depleted in the bud, and so, under sensitizing conditions (such as in the absence of FEAR network activity), cells are delayed in anaphase.