FIGURE 7:

The central domain of Lte1 binds to and inhibits Kin4. (A) Domain structure of Lte1 and position of the EcoRI sites that depict the boundaries of the EcoRI deletion allele. (B) Localization of GFP-tagged Lte1 (AS184) and GFP-tagged lte1-ΔEcoRI (AS351). Log-phase cells were fixed in 4% PFA and then imaged. Image acquisition conditions were the same for both strains. (C) Cells expressing GFP-tagged Kin4-S508A and Tub1-mCherry were arrested in G1 with 10 μg/ml α-factor pheromone. G1-arrested cells were then released into pheromone-free YEPD medium. Kin4(S508A)-GFP localization to SPBs was examined in late anaphase cells with the following genetic backgrounds: bub2∆ (A26874), bub2∆, lte1∆ (A26170), bub2∆, lte1∆, pLTE1-LTE1 (A38199), and bub2∆, lte1∆, pLTE1-lte1DecoRI (A38120). n ≥ 45. Error bars represent the range. (D) Wild-type (AS365) and lte1ΔEcoRI (AS367) cells containing the kin4(1-341)-GFP truncation were grown to log phase, and Kin4(1-341)-GFP localization was examined.