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. 2016 Dec 15;27(25):3991–4001. doi: 10.1091/mbc.E16-08-0563

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© 2016 Falk et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 8: — The GEF domains of Lte1 promote exit from mitosis. (A, B) Tenfold serial dilutions of overnight cultures of wild type (A2587), pGAL1-10-URL-3HA-LTE1 (A23686), KIN4-S508A (A21299), pGAL1-10-URL-3HA-LTE1 KIN4-S508A (A24084), pGAL1-10-URL-3HA-LTE1 KIN4-S508A pLTE1-lte1ΔEcoRI (AS336), pGAL1-10-URL-3HA-LTE1 KIN4-S508A pLTE1-LTE1 (A38247), spo12Δ (A4874), pGAL1-10-URL-3HA-LTE1 spo12Δ (A24543), pGAL1-10-URL-3HA-LTE1 spo12Δ pLTE1-lte1ΔEcoRI (AS338), and pGAL1-10-URL-3HA-LTE1 spo12Δ pLTE1-LTE1 (A38260) cells were spotted onto YEP plates containing either raffinose and galactose or glucose and incubated at 30°C. (C, D) pGAL1-10-URL-3HA-LTE1 (A23686), KIN4-S508A (A21299), pGAL1-10-URL-3HA-LTE1 KIN4-S508A (A24084), pGAL1-10-URL-3HA-LTE1 KIN4-S508A, pLTE1-lte1ΔEcoRI (AS336), pGAL1-10-URL-3HA-LTE1 KIN4-S508A pLTE1-LTE1 (A38247), spo12Δ (A4874), pGAL1-10-URL-3HA-LTE1 spo12Δ (A24543), pGAL1-10-URL-3HA-LTE1 spo12Δ pLTE1-lte1ΔEcoRI (AS338), and pGAL1-10-URL-3HA-LTE1 spo12Δ pLTE1-LTE1 (A38260) cells were grown overnight in YEP medium with raffinose and galactose to log phase and diluted into YEPD medium. Cells were grown for 6 h at 30°C, and samples were taken at the indicated times to determine the percentage of anaphase cells (n = 200). Note that the data for C and D are from the same experiment, and so the pGAL1-10-URL-3HA-LTE1 data from C are also shown in D.