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. 2016 Jun 14;127(1):179–185. doi: 10.1002/lary.26097

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© 2016 The American Laryngological, Rhinological and Otological Society, Inc.

This article is being made freely available through PubMed Central as part of the COVID-19 public health emergency response. It can be used for unrestricted research re-use and analysis in any form or by any means with acknowledgement of the original source, for the duration of the public health emergency.

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Sanger sequencing of Mycobacterium species. Sanger sequencing of the mycobacterium rpoB gene in iSGS scar demonstrating 99% positional identity with MtbC in eight of 20 iSGS samples. (A) Seven of the eight positive samples demonstrated two identical synonymous substitutions at positions 2312 and 2313. (B) Predicted identical rpoB amino acid sequence in MtbC and iSGS specimens. Analysis of rpoB DNA sequences from 29 Mycobacterium species and from patients with iSGS showing distinct clustering of iSGS samples. Phylograms based on nucleotide alignments were generated with HKY85 distances matrices using Paup 4.0b10 (Sinauer Associates, Sunderland, MA). (C) Bootstrap values > 50 (based on 500 replicates) are represented at each node. The branch length index is represented below the phylogram. iSGS = idiopathic subglottic stenosis; MtbC = mycobacterium tuberculosis complex.