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. 2016 Dec 8;7(1):1–11. doi: 10.1016/j.ijpddr.2016.12.002

Fig. 3.

Fig. 3

Photomicrographs of histological sections of the spleen and liver from golden hamsters infected with L. (L.) infantum and treated with 1.0 mg/kg or 2.0 mg/kg of ursolic acid (UA) and 5.0 mg/kg of amphotericin B. Macrophagic invasion in the white pulp of the spleen (WP) was observed in the infected control group (A), and to a lesser degree in the animals treated with 1.0 mg/kg (B) or 2.0 mg/kg (C) of UA and amphotericin B (D). In the non-infected control group (E), no alterations were found. In the red pulp of the spleen (RP), macrophagic nodules (arrow) were found in all infected groups (F-I), whereas non-infected control animals did not present such alterations (J). Inflammation foci (arrow) were verified in liver portal areas of the infected control group (K), as well as of the UA- (L and M) or amphotericin B- (N) treated hamsters. Non-infected animals (O) showed normal histological characteristics. In the liver parenchyma, macrophagic granulomas (arrow) were observed in the infected control group (P) and in animals treated with UA (Q and R) or amphotericin B (S), while the non-infected control group did not exhibit such alterations (T). Spleen histological sections (A-J), 400× magnification; scale bars: 20 μm (A-E) and 10 μm (F-J). Liver histological sections (K-T), 100× magnification; scale bars: 20 μm (K-O) and 10 μm (P-T).