Fig. 2. Tunicamycin (TM)-induced endoplasmic reticulum stress accelerates cell death in the human retinal pigment epithelial cell line, ARPE-19. (A) ARPE-19 cells were treated with vehicle or TM (1 µg/mL). After 48 hours, cell nuclei were stained to detect live cells using Hoechst 33342. (B) Quantification of the amount of live cells. Values are mean ± standard deviation, n = 6. *p < 0.05 vs. vehicle. (C) The viability of ARPE-19 cells was measured 48 hours after vehicle or TM (0.01, 0.1, and 1 µg/mL) treatment using the Ez-Cytox Cell Viability Assay Kit. Values are mean ± standard deviation, n = 12. *p < 0.05 vs. vehicle.
