Table 3.
Steady-State Kinetic Parameters for Hydrolysis activity of CTX-M-15 in presence of various drugs.
| Km (μM) | Kcat (s−1) | Kcat/Km (μM−1s−1) | |
|---|---|---|---|
| CTX-M-15 only | 88.13 ± 0.5 | 7618.97 ± 1.4 | 86.451 |
| CTX-M-15+ CTX | 65.932 ± 0.9 | 7618.97 ± 0.85 | 115.557 |
| CTX-M-15+ STR | 93.789 ± 1.0 | 6015.02 ± 0.3 | 64.133 |
| CTX-M-15+STR+CTX (Allosteric curve) | ~ 100 ± 2.0 | ~ 6285 ± 3.1 | ~ 62.85 |
| CTX-M-15+STR+FOX | 187.212 ± 1.6 | 8163.2 ± 2.5 | 43.604 |
| CTX-M-15+CTX+STR | 180.363 ± 1.8 | 10389.6 ± 0.4 | 57.603 |
| CTX-M-15+CTX+FOX | 304.45 ± 0.9 | 10389.6 ± 2.2 | 34.12 |
| CTX-M15+STR+FOX+CTX | 103.589 ± 0.6 | 5197.74 ± 1.3 | 50.176 |
| CTX-M-15+CTX+STR+FOX | 149.419 ± 0.2 | 6014.971 ± 0.7 | 40.255 |
| CTX-M-15+CTX+FOX+STR | 129.165 ± 2.1 | 6349.142 ± 1.5 | 49.155 |
CTX, cefotaxime; STR, streptomycin; FOX, cefoxitin.
Kinetic constants are reported as the average of ± standard error from three independent experiment.