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. 2016 Dec 15;7:567. doi: 10.3389/fphys.2016.00567

Figure 2.

Figure 2

TnI Ser-150, but not Ser-23/24, pseudo-phosphorylation decreases TnI binding to actin. Average solid-phase TnI-actin protein binding data demonstrating that compared to WT (TnI WT: black circle, solid line), the pseudo-phosphorylation of Ser-150 (TnI S150D: black Square, dashed line) and Ser-23/24/150 (TnI S23/24/150D: gray diamond, solid line) decrease TnI binding to actin while the pseudo-phosphorylation of Ser-23/24 (TnI S23/24D: gray triangle, dashed line) do not. Protein binding assays were conducted in triplicate wells and repeated.