FIGURE 3.

Phylogenetic inferences of 16S rRNA gene among bacteria isolated from DA and AO feces samples. A phylogenetic tree of 16S rRNA genes was constructed with the maximum likelihood (ML) method using a Tamura–Nei model distance matrix. The confidence levels were calculated from 1000 bootstrap resamples of alignment used for phylogenetic inferences by ML method. Black circles represent the nodes with a support bootstrap value of ≥40%. Numbers in brackets represent the number of corresponding excised, cloned, and sequenced DGGE band. Group I, including phylum Firmicutes and bacteria from the families Lactobacillaceae, Enterococcaceae, and Turicibacteraceae is denoted by full semicircular line. Group II consisting of bacteria belonging to phylum Firmicutes and Proteobacteria including families Lachnospiraceae, Oxalobacteraceae, and Succinivibrionaceae is denoted by dashed semicircular line.