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. 2016 Dec 15;7:1981. doi: 10.3389/fmicb.2016.01981

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Copyright © 2016 Alvarez, Badia, Bosch, Giménez and Baldomà.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Escherichia coli reference collection (ECOR) strains bearing tcpC gene increase TER in T-84 cell monolayers. The effect on TER of the indicated Escherichia coli strains from the ECOR collection was analyzed in T-84 monolayers in comparison with the probiotic EcN and the laboratory strain HB101. TER values were measured before and after 24 h incubation with cell-free supernatants (CF-SN) (2 mg protein/ml) collected from LB cultures (n = 3 independent biological replicates). Data are presented as percentage of increase in TER from the initial value. ^asignificance against untreated control cells (p ≤ 0.001).