FIGURE 3.

Gene expression levels of tight junction proteins in the intestinal epithelial cell lines T-84 (A) or Caco-2 (B). Cell monolayers were challenged for 4 h with COF-SN (0.5 mg/ml) or OMVs (0.1 mg/ml) from EcN (white bars), EcN tcpC::kan (doted white bars), ECOR63 (gray bars) or ECOR63 tcpC::kan (doted gray bars). Relative mRNA levels of the indicated proteins were measured by RT-qPCR, using β-actin as the reference gene. Data are presented as fold-change compared to untreated control cells (n = 3 independent biological replicates). ^aSignificance against untreated control cells (p ≤ 0.04); ^bsignificance tcpC mutant vs. wild-type (p ≤ 0.02).