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. 2016 Dec 15;7:482. doi: 10.3389/fphar.2016.00482

FIGURE 1.

FIGURE 1

Ang-(1-7) inhibits inducible nitric oxide synthase (iNOS) induction elicited by Ang II or IL-1β in human aortic smooth muscle cells (HASMC). (A) HASMC were stimulated with Ang II (100 nM) alone or in the presence of the AT1 receptor antagonist losartan (1 μM) for 18 h, after which iNOS levels were determined by Western blot and normalized to α-tubulin. (B) In another set of experiments, the impact of increasing concentrations of Ang-(1-7) (1 nM–1 μM) on iNOS induction by Ang II was tested. (C) The pro-inflammatory cytokine IL-1β (2.5 ng/mL) promoted iNOS induction, which was prevented by the IL1 receptor blocker anakinra (1 μg/mL). (D) As for Ang II, Ang-(1-7) prevented the induction of iNOS by IL-1β in a concentration-dependent manner. Results are expressed as mean ± SEM; n = 5. Representative blots are shown in the corresponding panels. ∗p < 0.05 vs. untreated cultures, #p < 0.05 vs. Ang II- or IL-1β-treated cultures, respectively.