FIGURE 3.

Ang-(1-7) reduces NF-κB activation via Mas receptors in HASMC. The activation of NF-κB induced after 18 h incubation with (A) Ang II (100 nM) or (B) IL-1β (2.5 ng/mL) was blunted by the antioxidant NADPH oxidase inhibitor apocynin (30 μM) and significantly reduced by Ang-(1-7) (100 nM), as determined by EMSA. The effect of Ang-(1-7) was prevented by the Mas receptor antagonist A779 (1 μM). The induction of iNOS by Ang II (C) or IL-1β (D) was abolished by the NF-κB inhibitor pyrrolidine dithiocarbamate (PDTC) (100 μM). Representative blot or EMSA images are shown in the corresponding panels. Results are expressed as mean ± SEM; n = 4. ^∗p < 0.05 vs untreated cultures, ^#p < 0.05 vs Ang II- or IL-1β-treated cultures, respectively.