FIGURE 4.

Ang-(1-7) reduces NADPH oxidase activity via Mas receptors. In HASMC, Ang-(1-7) (100 nM) reduced the stimulation of NADPH oxidase observed after 18 h incubation with (A) Ang II (100 nM) or (B) IL-1β (2.5 ng/mL), and this effect was blocked by the Mas receptor antagonist A779 (1 μM). The inhibition of NADPH oxidase by the antioxidant inhibitor apocynin (30 μM) prevented the induction of iNOS by Ang II (C) or IL-1β (D). Representative blots are shown in the corresponding panels. The NADPH oxidase activity is given as relative light units (RLUs)/μg protein/min. Results are expressed as mean ± SEM; n = 4. ^∗p < 0.05 vs untreated cultures, ^#p < 0.05 vs Ang II- or IL-1β-treated cultures, respectively.