Figure 1.

Fold-expressions of Western blot assay results on GH, soleus, and TA muscles. (A) Each bar represents the density level of Hif-1 and Pdk-1 relative to the density of control GH muscle. Triplicate measurements were obtained from 2 different animals. Therefore, each measurement indicates an average value of 6 different gels and a corresponding standard error. Comparisons of Hif-1α (detected at 120 kD) and Pdk-1 (detected at 48 kD) expression in ambient air (control), 15 h intermittent hypoxic (IH), and 15 h sustained hypoxic (SH) conditions for GH, soleus, and tibialis anterior. Density levels of Hif-1α and Pdk-1 proteins were first standardized to β-Actin, and then calculated relative to the control GH muscle. Standard errors for each measurement are displayed in error bars. (B) Ratios between Pdk-1 and Hif-1α protein concentrations (Pdk-1/Hif-1α) are presented for GH, soleus and TA muscles. The ratios Pdk-1/Hif-1α in hypoxic conditions are standardized to the ratios taken from each control muscle; thus, each ratio measured in control state is used as a denominator. Note that the Pdk-1/Hif-1α ratio was decreased after IH condition and elevated by sustained hypoxia compared to control state in GH muscle.