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. 2016 Dec 15;7:604. doi: 10.3389/fphys.2016.00604

Figure 2.

Figure 2

Electrophoresis on harvested single fibers of the geniohyoid, soleus, and anterior tibialis muscles. (A) SDS-PAGE gels for single fibers obtained from the GH muscle treated in 15 and 30 h sustained hypoxia. Reference bands for myosin heavy chain isoforms indicate, from the top MyHC IIa, IIx, IIb and I, in the last lanes as a ladder. Note that two bands in each lane for MyHC IIa and IIb proteins are clearly visible after sustained hypoxic conditioning. (B) The change in MyHC composition compared to the previously reported control (MyHC IIa) was statistically significant (P < 0.05, n = 80). (C) Control soleus muscle consists of mostly MyHC I-containing fibers with a few MyHC IIa-containing fibers. MyHC composition of the soleus muscle remains unchanged under both intermittent and sustained hypoxic conditions. (D) All single fibers harvested from TA muscles consisted of MyHC IIb containing fibers. MyHC composition of the TA muscle remains unchanged in both hypoxic conditions.