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. 2016 Dec 15;7:604. doi: 10.3389/fphys.2016.00604

Figure 3.

Figure 3

(A) Note shifted MyHC mRNA expressions toward glycolytic side in IH and SH hypoxia after Pdk-1 protein was augmented. mRNA expression for each MyHC after Pdk-1 over-expression was compared to the baseline state in control muscle. For MyHC IIx and IIb, each comparison between Baseline vs. PDK+ were statistically significant. MyHC IIb increased most, but IIx and IIa also increased. For MyHC IIx, 1.0 ± 0.19 vs. 1.37 ± 0.23 under control, 1.13 ± 0.22 vs. 1.62 ± 0.04 under IH, and 1.28 ± 0.05 vs. 1.45 ± 0.06 under SH were measured. For MyHC IIb, 1.0 ± 0.03 vs. 1.48 ± 0.04 under control, 1.44 ± 0.10 vs. 1.92 ± 0.34 under IH, and 1.61 ± 0.23 vs. 1.93 ± 0.08 under SH were measured. All comparisons (means ± SE, n = 3) differ significantly at least at P < 0.05. (B) Changes in Pdk-1 and Hif-1α mRNA concentrations in C2C12 myotubes in hypoxia. Effects of hypoxic conditions on the expression changes are significantly greater than those changes resulted from Pdk-1 augmentation in Pdk-1 measurements. After Pdk-1 was overexpressed in myotubes, Hif-1α mRNA expressions did not significantly change in either IH or SH condition.