Table 1.
Strains and plasmids.
| Strain or plasmid | Relevant characteristics or function | Reference or origin |
|---|---|---|
| E. coli strains | ||
| DH5α | F-, ϕ80dlacΔM15,Δ(lacZYA-argF)U169, deoR, recA1, endA1, hsdR17(rk-,m k+), phoA, supE44, λ -, thi1, gyrA96, relA1 | TransGen |
| S17-1 | Thi pro hsdR recA Tra+ | Simon et al., 1983 |
| P. aeruginosa | ||
| PAK | Wild-type P. aeruginosa strain | David Bradley |
| Δeno | PAK with deletion of eno gene | This study |
| Δeno/eno | Δeno complemented by a wild type eno gene driven by its native promoter | This study |
| PAK/pUCP20 | Wild-type PAK with plasmid pUCP20 | This study |
| Δeno/pUCP20 | Δeno mutant strain with plasmid pUCP20 | This study |
| Δeno/ahpB-pUCP20 | Overexpression of ahpB in the Δeno mutant | This study |
| Δeno/ahpC-pUCP20 | Overexpression of ahpC in the Δeno mutant | This study |
| Δeno/ahpBC-pUCP20 | Overexpression of ahpB and ahpC in the Δeno mutant | This study |
| Plasmids | ||
| pUC18T-mini-Tn7T-Gm | For gene insertion in chromosome; Gmr | Choi and Schweizer, 2006 |
| pTNS3 | Helper plasmid | Choi and Schweizer, 2006 |
| pEX18Tc | Broad-host-range gene replacement vector | Hoang et al., 1998 |
| eno-pUCT-mini-Tn7T-Gm | Plasmid with an eno gene driven by its native promoter for chromosomal insertion | This study |
| ahpB-pUCP20 | Overexpression of ahpB | This study |
| ahpC-pUCP20 | Overexpression of ahpC | This study |
| ahpBC-pUCP20 | Overexpression of ahpB and ahpC | This study |
| pDN19lacZΩ | Promoterless lacZ fusion vector; SprSmrTcr | Li et al., 2013 |
| PahpB-pDN19lacZΩ | ahpB promoter of PAK fused to promoterless lacZ on pDN19lacZΩ; SprSmrTcr | This study |
| PahpC-pDN19lacZΩ | ahpC promoter of PAK fused to promoterless lacZ on pDN19lacZΩ; SprSmrTcr | This study |