Table 2.
List of overlapping primers used for the amplificati1on of 2.9 kb nucleotide binding site-leucine-rich repeat (NBS-LRR) region of Pi9 gene using primer walking technique.
| S.No. | Fragments | Forward Primer (5′–3′) | Reverse Primer (5′–3′) | Tm (°C) | Size (bp) |
|---|---|---|---|---|---|
| 1 | Pi9_1 | ATCAGTCACAAAATAGACTGTCATG | AGAGCTGTCTTGCCTAAACCAC | 60.0 | 741 |
| 2 | Pi9_2 | GATGGGTGGTTTAGGCAAGACAG | CGACATCCTTAGTCGTCATC | 60.0 | 836 |
| 3 | Pi9_3 | TCGTCTAGTAGGTAGATGGATAGCAG | TACCTTCACACCGAATGATTCAG | 60.0 | 808 |
| 4 | Pi9_4 | ACCAAAGTTGCTGGTCTGAATCA | TCGTGATCCCTTCGGTCACTGTC | 60.0 | 791 |
| 5 | Pi9_5 | GTGCTGCGAATGGACAGTGACCGA | CTGATCTCATATTCCCTTCCACAAT | 60.0 | 742 |