Table 2.
Proteins identified from a miniaturized assay of HTN5 treatment of C. albicans with increased MS signals. Average MS signals and their standard deviations were calculated based on six replicates. Proteins indicated with an asterisk (∗) have been determined using their associated protein families. Lower case letters in amino acid sequences indicated modified residues. Blank spaces indicated proteins unidentified by the databases.
| Biological function | Observed precursor m/z | Theoretical precursor m/z | Ion counts | p values | Amino acid sequence | Protein name | Charges | X Corr | |
|---|---|---|---|---|---|---|---|---|---|
| Before | After | ||||||||
| Surface-associated | 1297.59 | 1298.54 | 18000 ± 8800 | 51000 ± 5400 | 0.00042 | K.KPsTEDtFSK.Y | Peroxisomal protein family∗
(A0A0A6JC81) |
1 | 1.82 |
|
| |||||||||
| Mitochondrial-associated | 1297.59 | 1297.49 | 18000 ± 8800 | 51000 ± 5400 | 0.00042 | R.NsIttEsVK.A | Aldehyde hydrogenase family∗
(A0A0A3BNV6) |
1 | 2.03 |
|
| |||||||||
| DNA binding | 575.44 | 576.45 | 9700 ± 1000 | 28000 ± 6200 | 0.00055 | K.SLLSR.I | Telomerase reverse transcriptase family∗
(C4YDL3) |
1 | 1.54 |
| 1297.59 | 1297.75 | 18000 ± 8800 | 51000 ± 5400 | 0.00042 | K.NVIPAtItK.V |
Spt6 (D3IZV2) |
1 | 1.54 | |
| 1298.67 | K.EFTIGPFKcIK.W | Transcriptional regulatory protein family∗
(A0A0A3CXJ5) |
1.56 | ||||||
|
| |||||||||
| Miscellaneous | 575.44 | 576.31 | 9700 ± 1100 | 28000 ± 6200 | 0.00055 | R.SLNSR.I | ATPase family∗
(A0A0A4CD62) |
1 | 3.15 |
| 1901.71 | 1903.74 | 7800 ± 3900 | 13000 ± 2300 | 0.025 | K.NKATssSSStsRDTR.W | Leucine rich repeats∗
(C4YMX3) |
2 | 3.19 | |
|
| |||||||||
| Uncharacterized | 1297.59 | 1297.69 | 18000 ± 8800 | 51000 ± 5400 | 0.00042 | R.NNTtVSKGRIK.V | (A0A0A6M207) | 1 | 1.51 |
| 1297.70 | K.KHQtHILNVK.S | (A0A0A6HZ28) | 1.60 | ||||||