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. 2016 Dec 15;7:597. doi: 10.3389/fphys.2016.00597

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Copyright © 2016 Feng and Jin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CAIII expression in neonatal and adult mouse TA, EDL and soleus muscles. (A) Glycerol-SDS-gel showed that MHC isoforms had similar contents and patterns of developmental switch in Car3-KO and WT TA and EDL muscles. (B) SDS-gels and Western blots showed that CAIII was expressed in neonatal soleus, TA and EDL muscles. The expression slightly lowered in soleus and ceased in EDL muscles during postnatal development. The expression of CAIIl in TA muscle diminished during postnatal development but re-activated in young adult. The expression of slow vs. fast troponin isoforms during postnatal development was examined using mAbs CT3 and TnI-1 Western blots and no difference was found between Car3-KO and WT muscles.