Figure 2.

Broad expression of CAIII in mouse skeletal muscles. (A) SDS-PAGE and mAb CP3 Western blots of multiple adult mouse skeletal muscles showed that CAIII is expressed at high levels in slow fiber-containing muscles and as well as in many pure fast fiber muscles. The relative slow and fast fiber contents of the muscle were estimated from the level of slow TnT detected in the mAb CT3 blots and the ratio of slow and fast isoforms of TnI in the mAb TnI-1 blots. (B) Glycerol-SDS-PAGE showed various MHC isoform contents in the fast fiber type mouse skeletal muscles studied without clear correlation with the expression of CAIII. (C) CAIII positive fast muscle TA and CAIII negative fast muscle EDL were chosen for functional comparisons.