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. 2016 Dec 15;7:1911. doi: 10.3389/fpls.2016.01911

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Copyright © 2016 Cho, Lee, Park, Choi and Kim.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Interaction specificity of TOC34 with CBLs in a yeast two-hybrid assay. Yeast cells (Y190 strain) were cotransformed with the combinations of the BD and AD constructs as indicated and selected on the synthetic complete media lacking Leu and Trp (SC-LW). Cotransformed yeast cells were cultured, serially diluted, and spotted onto the indicated media. Yeast growth on the synthetic complete media lacking His, Leu, and Trp (SC-HLW) indicates interaction.