Figure 7.

Inhibition of GTPase activity of TOC34 by Ca²⁺-bound CBL10. (A) The GTPase activity of purified GST-TOC34 protein. The GTPase activity is represented with the amount of free phosphate generated from the hydrolysis of GTP by the action of 4 μg of GST-TOC34 for 30 min at 27°C. GST protein was used as a negative control. (B) The effect of CBL10 or CBL4 on the GTPase activity of TOC34. The plus (+, 5 mM CaCl₂) and minus (−, 2mM EGTA) signs indicate the presence and absence, respectively, of the indicated components in the reaction samples. Each value represents the average of three independent experiments. Error bars indicate SD. Significance was calculated using the Student's t-test: ^*P < 0.05, ^**P < 0.01.