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Primer/probe sequences and concentrations (nM) used in quantitative real-time PCR (qPCR) assay.
| Sequence (5′-3′) | nt position∗ | Conc | |
|---|---|---|---|
| PED_S for | ACGTCCCTTTACTTTCAATTCACA | 1846–1869 | 500 |
| PED_S rev | TATACTTGGTACACACATCCAGAGTCA | 1931–1957 | 500 |
| PED_S Probe | FAM-TGAGTTGATTACTGGCACGCCTAAACCAC-BHQ1 | 1875–1903 | 200 |
∗According to the published sequence of the porcine epidemic diarrhea virus (PEDV) S1 gene, GenBank accession number DQ985739.1.
