Table 1.
Top Cntn6 interacting proteins.
| PROTID | Gene | FC-A | FC-B | SAINT | Protein | Cellular localization |
|---|---|---|---|---|---|---|
| P97528 | Cntn6 | 44.23 | 44.11 | 1 | Contactin-6 | Cell membrane |
| O94910 | LPHN1 | 16.87 | 13.16 | 0.88 | Latrophilin-1 | Cell membrane; Synapse |
| Q9UBS4 | DNAJB11 | 5.66 | 5.6 | 0.84 | DnaJ homolog subfamily B member 11 | Endoplasmic reticulum |
| O94779 | CNTN5 | 11.64 | 5.71 | 0.67 | Contactin-5 | Cell membrane |
| P27797 | CALR | 5.47 | 3.48 | 0.65 | Calreticulin | Endoplasmic reticulum; Cytoplasm, Extracellular space |
The proteins that were coimmunoprecipitated with Cntn6-TMGFPBio and detected by mass spectrometry are listed according their SAINT score. Data from triplicate IPs were compared to control using CRAPome, which employs SAINT and FC statistical analyses. This table lists the protein identification code (PROTID) and its related gene name, together with FC-A, FC-B, and SAINT scoring. The standard CRAPome Fold Change calculations (FC-A) estimates the background by averaging the spectral counts across the selected controls. Another more stringent Fold Change (FC-B) calculation estimates the background by combining the top 3 values for each identified interacting protein, while SAINT reports a probability of true interaction. Cellular localization was determined by searching Uniprot (UniProt Consortium, 2015). See the Supplementary Proteomics Datasheet for the complete raw data.