Table 1. Primers designed for the construction of pFB-1-sigma C plasmid and detection of viral RNA.
Names | Primer sequences (5′-3′) | Restriction sites |
---|---|---|
pFastBac-C F | CGGGATCCATGGATCGCAACGAGGTGATACGC | BamHI |
pFastBac-C R | GCTCTAGAGTGATGATGATGATGATGGCCCGTGGCGAC | XbaI |
Dia-F | GGAGTCGTCTCACTCCAAGC | |
Dia-R | TTTGCGAAGACATGAGCAAC |
Notes: The sequences in bold represent the corresponding restriction site, the underlined sequence indicates the 6 × His tag.