Table 2. Primers used for LR-PCR amplification of PLG.

PCR	PCR forward primer	Forward primer position*	PCR reverse primer	Reverse primer position*	Size (bp)
PLG_LR1	GCGCCAGCACAGAGCTCTGCTCAAC	chr6:161,121,602- 161,121,626	GCTTGCTACTTGTAAGAACTAATAACTTC	chr6:161,129,932-161,129,960	8,359
PLG_LR2	CAATTTAGCTCTCCAAACATTCTGCATCC	chr6:161,129,653-161,129,681	GAACTCCTTGAACTCACTCAAGCAATCC	chr6:161,137,405-161,137,432	7,780
PLG_LR3	AGGTACTAGATGAGTATCTTTAGGCAGG	chr6:161,137,170-161,137,197	GGCATCTCCATTGAGCTCACTGTTCC	chr6:161,144,785-161,144,810	7,641
PLG_LR4	GTCTTGCTGAACTGAGGAAGGAGACTGG	chr6:161,144,556-161,144,583	GTAGATGTCCAGCCACAGATTTCTTACC	chr6:161,152,264-161,152,291	7,736
PLG_LR5	CTGAATATTCTCCCACCTCTTGTGACC	chr6:161,152,034-161,152,060	GAACAGCTCTGTTCTGCAGTTTATTCAGG	chr6:161,159,857-161,159,885	7,852
PLG_LR6	CCCACTGCTTGGAGAAGTATGTTTAGG	chr6:161,159,629-161,159,655	TCAAGATCCAGGAGGATGGCAAAATCC	chr6:161,167,598-161,167,624	7,996
PLG_LR7	CATTTCTGAGATTCTTTCCTCAGCTTGG	chr6:161,167,413-161,167,440	TCAAAATGGGGAACAACATTTGTGTAAGG	chr6:161,175,204-161,175,232	7,820

^*Primer positions referred to GRCh37/hg19, UCSC Genome Browser assembly, February 2009 release (http://genome.ucsc.edu/)².