Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Dec 15;6:39049. doi: 10.1038/srep39049

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(A) Western blot analysis of REST and actin. HEK293FT cells were transfected with control and REST-V5 expressing vector, respectively. Levels of REST and actin were assessed by western blotting. (B) Immunofluorescence staining to confirm localization of transiently expressed REST. HEK293FT cells transfected with the REST-V5 expressing vector were fixed after 24 h, followed by probing with V5 antibody. Images analyzed at magnification of 400x. Scale bar indicates 10 μm. (C) Experimental procedures for LC-MS/MS analysis after immunoprecipitation of REST. V5-tagged REST proteins were expressed in HEK293FT cells and immunoisolated using anti-V5 antibody immobilized on agarose beads. Immunocomplexes were eluted and digested for LC-MS/MS analysis. (D) SDS-PAGE and Coomassie blue staining of immunocomplexes. The arrow indicates REST. Full-length gels and blots are included in the Supplementary Figure 3.