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. 2016 Dec 15;6:39049. doi: 10.1038/srep39049

Figure 6. Expression of CTNND2, REST and TRIM28 during the differentiation of primary neuron.

Figure 6

(A) Primary neurons were obtained from mouse embryo and maintained in the culture plate. The primary neurons were fixed on days 0, 2 and 4, followed by probing with Phalloidin. Scale bar indicates 50 μm. (B–D) mRNA levels of REST (B), TRIM28 (C) and protein levels of REST, TRIM28 (D) were accessed in the primary neurons on days 0, 2 and 4. (E) mRNA level of CTNND2 accessed in the primary neurons on days 0, 2 and 4. Three independent experiments were performed to analyze alteration of genes at mRNA level, and the t-test was used for statistical analysis. (*P < 0.05; **P < 0.01; ***P < 0.001).