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. 2016 Nov 3;594(24):7197–7213. doi: 10.1113/JP272988

Figure 2. Validation and optimisation of the muscle fibre seeding procedure.

Figure 2

A–D, representative examples of intact single extensor digitorum longus (EDL) muscle fibre (A–C) and small EDL fibre bundles (D). Scale bars: 50 μm. Seeding densities were optimised using single EDL fibres and EDL fibre bundles. E, basal oxygen consumption rate (OCR) readings from single fibres (n = 11), 2–3 single fibres (n = 14), 4–5 single fibres (n = 14) and 7–10 single fibres (n = 15). Wells containing 4–5 single fibres yielded an optimal basal OCR reading between 100 and 200 pmol min–1. F, basal OCR readings from 4–5 single EDL fibres (n = 14) and small EDL fibre bundles containing 4–5 myofibres (n = 15). The use of small EDL fibre bundles resulted in optimal basal OCR with less variance than multiple single fibres. G, the fluorescence intensity of the alamarBlue cell viability assay was linearly correlated with EDL fibre numbers (total n = 94). [Colour figure can be viewed at wileyonlinelibrary.com]