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. 2016 Nov 3;594(24):7197–7213. doi: 10.1113/JP272988

Figure 3. Optimisation of the mitochondrial stress test using extensor digitorum longus (EDL) muscle fibre bundles.

Figure 3

A, basal oxygen consumption rate (OCR) readings of EDL fibre bundles remained stable throughout the 80 min of measurement in response to three injections of assay medium (n = 5). B, optimal inhibition of ATP‐coupled OCR in EDL fibre bundles by oligomycin A (Oligo, 0.25‐2.0 μm), followed by injections of carbonyl cyanide‐p‐trifluoromethoxyphenylhydrazone (FCCP, 0.4 μm) and antimycin–rotenone (AA/ROT, 1.0 μm). C, baseline normalised OCR levels at the sixth measurement cycle (M6), after different working concentrations of oligomycin A were injected to inhibit ATP synthesis in EDL fibre bundles. Oligomycin A at 0.25 or 0.5 μm did not decrease the OCR when compared with the basal OCR, while oligomycin A at a working concentration of 1.0 μm or above induced a significant inhibition of OCR in EDL fibre bundles (oligomycin A groups: 0.25 μm, n = 9; 0.5 μm, n = 12; 1.0 μm, n = 10; 1.5 μm, n = 5; 2.0 μm, n = 6; * P < 0.05, compared with baseline (B)). [Colour figure can be viewed at wileyonlinelibrary.com]