Skip to main content
. 2016 Dec 14;36(50):12598–12610. doi: 10.1523/JNEUROSCI.1994-16.2016

Figure 5.

Figure 5.

Dynamics of GIFT15 Breg biodistribution. GIFT15 Bregs ameliorate EAE, and induce in vivo formation of CD25+FoxP3+ Tregs and CD49b+CD223+ Tr1s in mice with EAE. A, Real-time IVIS imaging with GIFT15-luciferase-Bregs or luciferase-B cells. MOG-immunized C57BL/6 mice were injected with 5 × 106 B6-L2G85-GIFT15 Bregs or B6-L2G85 B cells, and they were imaged every day for 2 weeks. B, Flow cytometry plot of GFP-GIFT15 Bregs or GFP-B cells after adoptive transfer in EAE C57BL/6 mice. Immunized mice were injected with 5 × 106 cells. After 7 d, the animals were killed (n = 5), and spleens, MLNs, and CNSs were collected. Leukocytes were isolated and analyzed by flow cytometry. Data are representative of two independent experiments with n = 5 mice in each group.