Figure 6.

GIFT15 B_regs ameliorate EAE and induce in vivo formation of CD25⁺FoxP3⁺ T_regs and CD49b⁺CD223⁺ Tr1s in mice with EAE. A, The evolution of clinical score in MOG_35–55-immunized Vert-X mice treated with GIFT15 B_regs or B cells (2 × 10⁶ cells/mouse, n = 10 per group). Data are reported as the mean ± SEM. The data are representative of two different experiments. B, Two-parameter contour plots show CD3 vs CD4, T_reg (CD25 vs FoxP3), and Tr1 (CD49b vs CD223) expression in spleen and MLN after 1 week of GIFT15 B_reg or B-cell adoptive transfer in EAE mice. The gating strategy was as follows: the lymphocytes were gated according to their side-scatter and forward-scatter properties, and CD4⁺ cells were gated from lymphocytes. CD4⁺ cells were stained for T_reg or Tr1 phenotype. GFP/IL-10 vs SSC is the frequency of either T_regs or Tr1s, respectively. C, Bar graphs are the mean ± SD values of two independent experiments (biological replicates) with n = 6 per group. p Values were calculated using the two-tailed Student's t test: *p < 0.05; **p < 0.01; ***p < 0.001.