Figure 5.

Inhibition of Hep^A abrogates changes in gene expression. RT–PCR and western blot were employed to confirm our results from the gene array using selected pro- and anti- apoptosis genes, n = 5–8 (Fig. 5A–D). Vinculin was used as a loading control, NC-negative control. In a separate experiment, cardiomyocytes were pre-treated with or without 125 μg/mL SST0001 for 4 h, prior to incubation with 500 ng/mL myc-Hep^L for 12 h, and the expression of selected genes determined, n = 4–9 (Fig. 5E). *P < 0.05, **P < 0.01, ***P < 0.001.