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. Author manuscript; available in PMC: 2016 Dec 15.
Published in final edited form as: Cell. 2016 Mar 24;165(1):125–138. doi: 10.1016/j.cell.2016.02.044

Figure 5. Elevated BAT Myostatin Expression Promotes Insulin Resistance.

Figure 5

(A) Insulin tolerance test in hM3DGqAgRP and hM3DGqWT mice (n = 7 versus 10) following co-injection of a selective β3-adrenergic agonist (CL 316,243) and CNO, or CNO and vehicle (saline).

(B and C) Representative blots and quantification of pAKT (B) and pSMAD 2/3 (C) from protein lysates of cultured, immortalized brown adipocytes treated with recombinant myostatin in the presence, or absence, of exogenous insulin at the indicated concentrations (n = 3–4 independent experiments).

(D) Insulin tolerance test in CNO-treated hM3DGqAgRP and hM3DGqWT mice 12 hr following subcutaneous injection of myostatin neutralizing antibodies (LSN2478185) or isotype control (n = 7 versus 10 for each treatment). Data are represented as mean ± SEM. *p ≤ 0.05, **p ≤ 0.01, and ***p ≤ 0.001 as determined by one-way ANOVA followed by Newman-Keuls post hoc test.