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. Author manuscript; available in PMC: 2016 Dec 15.
Published in final edited form as: Res Rep Biochem. 2016 Jul 7;2016(6):39–55. doi: 10.2147/rrbc.s107070

Figure 3. Adhesion of Mac-1-HEK293 and wild-type HEK293 cells to the LL-37-derived peptide K18-37.

Figure 3

(A) Aliquots (100 μl; 5×104/ml) of Mac-1-HEK293 cells were labeled with calcein and added to microtiter wells coated with different concentrations of K18-37. After 30 min at 37 °C, nonadherent cells were removed and adhesion was measured. Adhesion was expressed as percent of added cells. The data shown are means and S.E. from four experiments with triplicate determinations at each point. (B) Calcein-labeled Mac-1-HEK293 cells were incubated with different concentrations of K18-37 (●) or control peptide (▼) for 15 min at 22 °C and added to wells coated with 2.5 μg/ml fibrinogen and post-coated with 1% PVP. Adhesion (left ordinate) was determined as described in Fig. 2A legend. Right ordinate, Mac-1-HEK293 cells were treated with different concentrations of K18-37 for 15 min at 22 °C. Cells were centrifuged and fluorescence of cell supernatants determined (○). The data shown are the mean ± S.E. from two experiments each with triplicate determinations.