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. Author manuscript; available in PMC: 2016 Dec 15.
Published in final edited form as: Res Rep Biochem. 2016 Jul 7;2016(6):39–55. doi: 10.2147/rrbc.s107070

Figure 5. Binding density of LL-37 on the surface of mammalian and microbial cells.

Figure 5

Cell suspensions were incubated with 5 μg/ml 125I-LL-37-GY for 20 min at 22 °C. Non-bound peptide was removed by centrifugation at 200g (mammalian cells) or 2000g (bacteria) for 10 min. The pellet was washed with PBS, re-suspended in 100 μl PBS and radioactivity was measured. The adsorption of 125I-LL-37-GY to Immulon wells is described in the legend to Supplemental Fig. 1. The data shown are the mean ± S.E. from 3 experiments each with duplicate measurements.