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. Author manuscript; available in PMC: 2017 Sep 15.
Published in final edited form as: J Immunol. 2016 Aug 10;197(6):2208–2218. doi: 10.4049/jimmunol.1502575

FIGURE 3.

FIGURE 3

Differential Treg frequencies sustained over time. BALB/c mice were immunized with OVA/CFA, OVA/Alum, or OVA/Sm Eggs and cells isolated from the inflamed skin on days 5, 7, 10 and 14. (A) Foxp3+ frequency of CD4+ cells and (B) cell number of polyclonal Foxp3+ Tregs in the skin. (C) Ratio of polyclonal Treg:Tconv cell number in skin. Data is representative of two independent experiments with 3–4 mice per group. Statistics by Two-Way ANOVA, p=<0.001 for adjuvant comparison, and bonferroni post test shown for Alum versus Sm Egg. Additional statistics: (A) *** CFA versus Sm Eggs d7, d10; (C) ** CFA versus Sm Eggs d7, d10.