Treg phenotype in the distinct inflammatory milieus. (A, C, D) Gene expression analysis by TLDA of Foxp3-GFP+CD4+7AAD- Tregs isolated from inflamed skin d7. LN calibrator and normalized to GAPDH. Genes clustered on log2 of 2−ΔΔCt (−ΔΔCT) using Euclidean distance and average linkage. Numbered groups represent Tregs isolated from individual experiments. (B, D, F) Relative gene expression of select genes. Statistics by Mann-Whitney * p=<0.05 for LN and at least one of the inflamed skin groups, 3–5 independent experiments.