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. Author manuscript; available in PMC: 2017 Sep 15.
Published in final edited form as: J Immunol. 2016 Aug 10;197(6):2208–2218. doi: 10.4049/jimmunol.1502575

FIGURE 9.

FIGURE 9

Cognate-antigen increases Treg accumulation and upregulation of CD25. (A) CD25 MFI and (B) ST2 MFI of all transferred DO11.10+ Tregs in the skin of mice immunized with cognate-Ag (OVA) or non-cognate Ag (KLH). KLH data from Figure 7. (C) CD25 expression on DO11.10+ Tregs in KLH immunized mice with CFA or Sm egg adjuvants. Mice were pulsed with PBS (+PBS) or OVA peptide (+pOVA) i.v. 6 h prior to cell harvest. % of Treg cells in CD25hi gate. (D) DO11.10+ Tconv cells transferred alone, CD25 MFI of all Tconv cells in the skin d7 post-immunization. (E) rIL-33 (2μg/ml) was administered i.p. to CFA or Sm-Egg-inflamed (KLH or OVA) mice on days 4, 5 and 6 post-immunization and tissues analyzed on day 7. Expression levels for CD25 in the presence or absence of rIL-33 and non-cognate Ag (KLH) or cognate Ag (OVA). Data representative of 2–3 independent experiments. Statistics by T Test and Mann-Whitney.