Figure 3.

Both PBMC and PBMC/PMN cocultures respond to anti‐CII IC but not to control IC in a TLR4‐dependent manner. (A and B) Effect of TLR4 blockade on the production of (A) TNF‐α and (B) CXCL8 from PBMC and PBMC + PMN cocultures (CoCulture) stimulated with anti‐CII IC. (C and D) Effect of TLR4 blockade on the production of (C) TNF‐α and (D) CXCL8 from PBMC and PBMC + PMN cocultures (CoCulture) stimulated with plate bound IgG (P‐IgG). Significance levels are shown between cell cultures treated with anti‐TLR4 antibodies or with control rabbit IgG (ctrl Ab). Data are from the eight donors also depicted in Figure 1C and D. Data were obtained in four experiments with two donors each (total eight donors). Paired investigations were made with the Wilcoxon test.