Figure 4.

Upregulation of CXCL8 in anti‐CII IC‐stimulated cocultures is suppressed by selectively blocking anti‐TLR4 on PBMC (A and B) Effect on the production of (A) TNF‐a and (B) CXCL8 of selectively blocking TLR in the PBMC and PMN coculture compartments. PBMC and PMN were individually treated with anti‐TLR antibodies (TLR4) or control antibodies (ctrl) and thereafter washed before being merged into cocultures (CoC). In the first comparisons from the left both PBMC and PMN were treated with anti‐TLR4 (P + N), in the second only PBMC (P) and in the third only PMN (N). The following comparisons are controls, showing that the individual treatment/washing steps did not change the overall effect of anti‐TLR4 blockade in cocultures. Comparison was done also with PBMC cultures (P), to show that the experimental conditions yielded the awaited upregulation in anti‐CII IC‐stimulated cocultures. Data were obtained in two experiments with two donors each (total four donors).