Figure 7.

Upregulation of CXCL8 in anti‐CII IC‐stimulated cocultures depends on high anti‐CII antibody density and is not seen for other IC. Effect of antibody density on the difference between PBMC and PBMC + PMN cocultures (Co‐culture) in production of TNF‐α and CXCL8. (A and B) Stimulation was done with anti‐CII containing IC (anti‐CII IC) with increasing antibody concentrations and responses were evaluated as production of (A) TNF‐a and (B) CXCL8. (C and D) Stimulation was performed with surface‐bound IC made with tetanus toxoid and anti‐TT antibodies (TT‐IC) and evaluated as production of (C) TNF‐a and (D) CXCL8. (E and F) Stimulation was performed with directly coated IgG (plate‐bound IgG) and evaluated as production of (E) TNF‐a and (F) CXCL8. Antibody density in all ICs had been normalized against a standard curve consisting of plate‐bound IgG as described in 17. Eight micrograms per milliliter of anti‐CII represents the highest anti‐CII concentration available. The results are from one of two experiments with similar results.