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. 2016 Dec 6;16(23):2937–2944. doi: 10.1002/pmic.201600341

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© 2016 The Authors. Proteomics Published by Wiley‐VCH Verlag GmbH & Co. KGaA, Weinheim.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Extraction efficiency evaluation of the different protocols using SDS‐PAGE based purity ratios. (A) Purity of histone extracts prepared using Protocols A, B, and C on fresh and snap‐frozen cells for Jurkat cells and hESC, as compared to bovine histones, a commercial extract obtained after extensive fractionation. Independent technical replicates n = 3, mean ± SD. Replicates were prepared in different tubes at different time points by the same person. (B) Four protocols were selected for subsequent MS analysis and performed on Jurkat cells.