Figure 1.

PYL1 is required for the increased FUS3 stability at high temperature (A) Germination rates of wild type (WT) and pyl1-1 T-DNA insertion mutant (SALK_054640; Guzman et al. 2012) imbibed at control (21°C), supraoptimal (32°C) temperatures, or 21°C + 0.5 micromolar ABA. 3 months-old seeds were sterilized and imbibed on half strength MS plates as previously described (Chiu et al. 2016). Averages from triplicates ± standard deviation (SD) are shown. (B) Immunoblots showing degradation of GST-FUS3 in cell extracts of 3 DAI WT and pyl1-1 seeds imbibed at 32°C. Cell free degradation assays were performed as previously described (Chiu et al. 2016). Immunoblots were probed with 1:1000 anti-GST antibody (one representative is shown). Ponceau S stain is shown as a loading control. A plot showing the quantification of GST-FUS3 levels in 3 biological replicates ± SD is shown.