Figure 2.

GA accelerates FUS3 degradation at 32°C (A) Germination rates (% radíele protrusion) and seedling growth (% cotyledon expansion) of wild type (WT) seeds imbibed at 32°C in the presence or absence of 10μM GA. Six-months-old dry seeds were sterilized and ¡mbibed on MS/Agar plates as previously described (Chiu et al., 2016). Averages from triplicates ± standard deviation (SD) are shown. (B) Immunoblots showing GST-FUS3 degradation rates in cell extraets of 3 DAI WT seeds imbibed at 32°C in the presence or absence of 10μM GA. Cell free degradation assays were performed as previously described (Chiu et al., 2016). Immunoblots were probed with 1:1000 anti-GST antibody and Ponceau S stain is shown as a loading control. A plot showing the quantification of GST-FUS3 levels in 3 biological replicates ± SD is shown.