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Strategy for CRISPR/Cas9 mediated cleavage of the HIV-1 genome. Guide RNAs (gRNAs) targeting the U3 region of the LTR can recruit Cas9 to the viral DNA sequence integrated in the host chromosome and results in cleavage of the viral DNA at the designated sites and introduces InDel mutations. Cleavage at both the 5′ and 3′ LTRs can lead to removal of the complete coding region of HIV-1 from the host genome and lead to eradication of HIV-1 from the host [133, 134].
