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TrpCN fluorescence decay kinetics of M2TM-WCN in 100 nm vesicles composed of POPC, POPG, and cholesterol (4:1:2) at pH 5.0 and 7.4, as indicated. In each case, the smooth line corresponds to a fit of the kinetics to a double-exponential function with the following time constants (relative amplitudes): 7.8 ns (0.62) and 2.5 ns (0.38) for pH 5.0; 7.5 ns (0.62) and 2.4 ns (0.38) for pH 7.4. Top panel shows the residuals of the fits.
